Live Event
Streamlined High Pressure Freezing for Cryo-ET: Waffle Method to Serial Lift-Out
June 25, 2026 04:00 PM (London)
Georg Wolff
Georg earned his PhD at Leiden University Medical Center in 2022, developing cryo-electron tomography workflows to study virus replication in cells. He then joined EMBL and worked on cryo-FIB milling and correlative cryo-fluorescence microscopy, especially of high-pressure frozen samples. Since 2024, he has been a Cryo-Electron Tomography Specialist at the EMBL Imaging Centre, making state-of-the art cryo-EM workflows available for the broader research community.
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Christoph Kaiser
Christoph is a scientist and microscopy specialist at the Max Planck Institute of Biochemistry. Trained in biochemistry at the Technical University of Munich, with expertise in structural biology workflows, his research focuses on cryo-electron tomography, correlative microscopy, and cryo-focused ion beam sample preparation, especially Serial Lift-Out workflows for multicellular organisms and tissues. He combines interdisciplinary approaches to enable the study of quasi-native complex biological systems across scales.
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Miriam Sarah Weber
Miriam is a Postdoctoral Scientist at the University of Zürich, specializing in cryo-ET sample preparation. Her research focuses on making high-pressure freezing workflows more practical and accessible, including the development of Autogrid-compatible approaches. By simplifying handling and improving workflow integration, her work helps enable more reliable sample preparation for downstream cryo-FIB milling and tomographic analysis of complex biological samples.
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Andreia Pinto
Andreia started her career as a Biomedical Scientist in Histocellular Pathology, with a particular interest in electron microscopy. She has worked at the Primary Ciliary Diagnosis in Lisbon and the Royal Brompton Hospital in London. In 2022, she completed her PhD on the use of deep learning to analyze EM images of cilia for PCD diagnosis, while also studying SARS-CoV-2 infection of the respiratory airway. She is currently an Application Workflow Specialists Manager with Leica Microsystems.
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Discover how streamlined HPF sample preparation and scalable lift-out workflows can reduce handling, improve reproducibility, and advance cellular cryo-ET of complex specimens.
In this webinar, you will:
Explore practical refinements that make HPF-enabled cryo-ET workflows more robust, reproducible, and scalable
Learn how simplified planchette-based Autogrid freezing can reduce handling and support downstream cryo-FIB milling
Discover how Waffle Method preparation and Serial Lift-Out can improve throughput and contextual sampling from complex specimens
High pressure freezing (HPF) is opening cryo electron tomography (cryo ET) to thicker, more complex specimens—but many labs and facilities still face fragile, time intensive workflows that slow routine adoption. In this webinar, four speakers share practical approaches to make HPF-enabled cryo ET more robust, reproducible, and scalable.
You’ll hear how targeted, “small” technical refinements can streamline cellular cryo tomography of HPF specimens prepared by the Waffle Method or in planchettes—especially when processing diverse samples at service facility scale.
Next, learn about a simplified HPF workflow that freezes clipped EM Autogrids directly in planchettes designed for Autogrids, reducing post-freezing handling and supporting downstream cryo FIB milling for on-grid lamellae and lift out.
A third talk introduces Serial Lift-Out—an enhanced lift out approach that prepares multiple sections/lamellae from a single transfer to increase throughput and contextual sampling in multicellular specimens.
Finally, Leica Microsystems will present the high pressure freezer EM ICE for cryo EM applications and show how to implement HPF for the Waffle Method as a consistent starting point for cryo FIB lamella preparation and cellular cryo ET.
Feature Image: Serial Lift-Out workflow for cryo-ET lamella preparation - Schematic illustration of the Serial Lift-Out method applied to a vitrified biological sample embedded in bulk vitreous ice (blue). Image courtesy: Prof. Dr. Jürgen M. Plitzko - doi.org/10.1038/s41592-023-02113-5
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