Live Event
See the Hidden: Adding time to the correlative equation
October 17, 2023 01:00 PM (London)


Paul Verkade
Dr. Paul Verkade is a champion of Correlative Microscopy and is passionate about training the next generation of microscopists. He has taught and organized numerous RMS and other courses, including 4 EMBO practical courses on CLEM in Bristol, and edited 5 books on Correlative Microscopy. He is a long-standing member of the Electron Microscopy section of the RMS, having served as chair and other functions. He has been involved in the set-up of the RMS mentoring scheme that started in 2022. He focuses on bringing together imaging communities and organizing the funding of imaging infrastructure.
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Nalan Liv
Nalan Liv leads a cellular nanoimaging and organelle biology research team and coordinates the Cell Microscopy Core at UMC Utrecht, the Netherlands. Her scientific niche is positioned at the interface of cell biology and advanced microscopy. She develops correlative fluorescence and electron microscopy approaches and applies them to resolve organelle functions and alterations in cancer cells. Nalan’s research interests include endo-lysosomal biology, lysosomes in cancer, correlative microscopy, live-cell imaging, and volume electron microscopy; she has co-authored more than forty papers and given invited microscopy and cell biology talks around the world. Nalan is an active member of the international microscopy community and is committed to the growth of the field through collaboration.
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Joachim Greiner
Joachim Greiner is a researcher in the 4D Imaging and Computational Modelling group at the Institute for Experimental Cardiovascular Medicine (IEKM) at the University of Freiburg, Germany. His research involves spatial and temporal characterization of cardiomyocytes, cardiomyocyte isolation (machine learning-aided) image analysis, and computational models of cardiac conduction. In his study of the ultrastructural dynamics of cardiomyocytes, he employs the Leica EM ICE, utilizing action potential-synchronized high-pressure freezing and electron tomography.
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Jana Kroll
Dr. Jana Kroll is a postdoctoral research fellow in Neuroscience and Structural Biology at the Max Delbrück Center for Molecular Medicine and Charité-Universitätsmedizin Berlin. Her research focuses on the structural and functional analysis of molecular machinery mediating neuronal communication using in situ cryo-electron tomography (cryo-ET). Jana obtained her doctoral degree from the Georg August University of Göttingen, and her expertise encompasses techniques like plunge freezing, high-pressure freezing, cryo-confocal correlative microscopy, and 3D segmentation for cryo-ET. Her research contributions are reflected in her publications that illuminate the intricate workings of neuronal exo- and endocytosis.
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Frederic Leroux
Dr. Leroux completed his Master's degree in Biology at the University of Ghent, where he gained experience in biological EM sample preparation. During his Ph.D. at the EMAT research group, University of Antwerp, Frédéric specialized in advanced electron microscopy of composite materials, becoming an EM sample preparation specialist at EMAT. In 2016, he joined Leica Microsystems as an Application Specialist in Nanotechnology, using his multidisciplinary background and broad microscopy experience to improve EM sample preparation for various materials (polymers, composites, biological and industrial materials).
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Gareth Jackson
Gareth Jackson is an Advanced Workflow Specialist and Product Sales Specialist at Leica Microsystems. With 20+ years of electron microscopy experience, he specializes in EM sample preparation workflows for various systems, including cryo-EM and correlative imaging techniques for cellular imaging. Gareth started his career in clinical EM and has since developed a broad range of experience in microscopy and EM sales and applications through roles at Olympus and Gatan as well as Leica Microsystems. Gareth is an active member of the EM community and is regularly involved in courses and workshops in the UK.
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Learn how to stimulate and synchronize subcellular events using light and electricity and discover workflow solutions for correlative imaging that enable time stamping of dynamic biological events during high-resolution EM imaging.
In this webinar, you will learn:
- Methods to trigger subcellular events with light or electrical stimulation, and capture them with CLEM;
- How to use correlative EM to extract three-dimensional data within a temporal context;
- About Leica's Coral Life Live Cell CLEM workflow solution and how it can timestamp dynamic biological events.
In the next virtual edition of our See the Hidden Workshop series, we show you how Leica’s workflow solutions for correlative imaging enable you to add a timestamp to a dynamic biological event while capturing it with high-resolution EM imaging.
With the help of leading researchers, we explore how CLEM workflows can help capture dynamic events, such as neurotransmission at the synapse, and how optical and electrical stimulation can trigger and synchronize cellular processes right before vitrification.
The first session focuses on Live Cell CLEM workflows, where cutting-edge techniques open doors to observing dynamic cellular processes. Featuring renowned experts Paul Verkade, Nalan Liv, and Leica’s Frédéric Leroux, this session will shed light on the convergence of live cell imaging and electron microscopy.
The second session will emphasize using CLEM workflows involving light and electrical stimulation. Correlative imaging experts Joachim Greiner and Jana Kroll will guide you through innovative methods and their latest research findings.
Discover how such approaches allow researchers to trigger and capture subcellular events such as neuron endocytosis with exceptional precision. Both sessions will conclude with an interactive panel discussion between the speakers.
AGENDA
13:00 GMT | 14:00 CET — Session 1: Live Cell CLEM workflows
Scientific Presentations followed by live Q&A:
- Paul Verkade, University of Bristol, UK
- Nalan Liv, UMC Utrecht, The Netherlands
- Frédéric Leroux, Leica Microsystems, Belgium
14:40 GMT | 15:40 CET — Coffee Break
14:50 GMT | 15:50 CET — Session 2: Capturing Ultra-Fast Processes
Scientific Presentations followed by live Q&A:
- Joachim Greiner, Universitäts-Herzzentrum Freiburg, Germany
- Jana Kroll, Max Delbrück Center for Molecular Medicine, Germany
- Frédéric Leroux, Leica Microsystems, Belgium
16:00 GMT | 17:00 CET — Closing Remarks
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