Optimising CRISPR Mouse Model Pipelines — Modified Synthetic sgRNAs
On-demand
Matthew Mackenzie
Matthew Mackenzie earned a BSc in Genetics at the University of Liverpool, where he spent a year in industry with LGC Forensics (now Eurofins) gaining insights into high-throughput sample analysis and automation. After graduating in 2015 he joined Oxford BioMedica, where he honed his molecular and cellular biology skills whilst developing inducible stable cell lines for the production of lentiviral vectors. Joining the Molecular and Cellular Biology group at MRC Harwell in 2016, he now works as part of a team to provide genome engineering and validation services for the generation of novel genome edited mouse models.
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In this webinar, you will see:
- The optimization of the mouse model generation workflow.
- A direct comparison of in vitro transcribed vs. modified synthetic single-guide RNA (sgRNA).
The development of CRISPR/Cas9 tools has revolutionized the genome-editing field, allowing for the generation of genetically modified mice with increased efficiency and ease. We provide a genome engineering service to produce and validate various knock-out (KO) and knock-in models through our evolving processes. This service has led to the development of a high throughput pipeline for the efficient generation of KO mice, allowing the generation of more than 100 KO projects a year as part of the International Mouse Phenotyping Consortium effort.
Additional pipelines include the generation of bespoke genome-edited mouse models, both as an on-demand service and as part of the Genome Editing Mice for Medicine service. One optimization in our pipeline was the transition from generating sgRNAs in-house using in-vitro transcription to buying in synthetic sgRNAs. We assessed the impact this had on our workflow, and we share these findings in our webinar.
Discover more about CRISPR in the Bitesize Bio CRISPR Research hub.
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