Linking 2D and 3D Spatial Biology for Multiscale Mapping of Complex Tissues

Explore how to bridge 3D imaging and 2D multiplexing to map complex tissues such as glioblastoma and gain deeper insight into brain biology and cancer progression.

In this webinar, you will:

• See how intact tissues can be visualized in 3D with light-sheet microscopy (while preserving spatial integrity)
  
  
• Explore how high-plex MICS technology supports single-cell proteomic profiling
  
  
• Learn how light sheet-guided histology enables precise ROI selection for a novel 3D–2D workflow

Discover how combining whole-organ light-sheet imaging with high-plex spatial proteomics enables multiscale mapping of tissue architecture and molecular phenotypes within the same specimen.

Using a glioblastoma mouse model, this webinar will highlight how linking volumetric imaging with targeted multiplexing unlocks deeper insights into brain biology and cancer progression.

A key highlight of the session will be Harold Cremer, Research Director at the Developmental Biology Institute of Marseille, who will discuss how 3D imaging and spatial biology approaches can advance our understanding of brain tumor development.

Kevin Bigott, M.Sc., Team Coordinator in R&D Neurobiology at Miltenyi Biotec, will present the underlying workflow innovations, from 3D labeling and clearing to light sheet-guided sectioning for 2D high-multiplex phenotyping.

Together, they will show how integrating technology and biology enables a new level of spatial insight across scales.