End-Point PCR Set-Up and Optimization: Best Practice
Broadcast date: December 05, 2023On-demand
Dr. Steffen Riethmüller
Dr. Steffen Riethmüller studied technical and applied biology in Bremen and wrote his master's thesis at the Max Planck Institute in Magdeburg. During his PhD at the Institute of Biochemistry in Kiel, he characterized the soluble IL-6R, associated with chronic inflammatory diseases, with the help of PCR and other techniques. Before joining Eppendorf, he was a Postdoctoral researcher at the Pharmaceutical Institute in Kiel.
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Join us for an eye-opening discussion of the factors that influence PCR results, including reaction set-up, consumables, and pipetting technique, and learn how to optimize them for better results.
In this webinar, you will learn:
- The important features of a thermal cycler.
- Ideal PCR set-up including primer design, template DNA selection, and PCR optimization.
- Good PCR pipetting technique and how to minimize evaporation.
Master your PCR set-up for unparalleled results. Join us for an enlightening discussion of crucial factors that influence the outcome of PCR experiments and discover the parameters you can optimize for better and more consistent PCR results.
Get reliable advice directly from PCR experts at Eppendorf on key topics, including the core features of thermal cyclers and the importance of good pipetting techniques to minimize evaporation.
Plus, gain helpful insights into ideal reaction set-up, primer design, template DNA selection, and PCR optimization.
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