Challenges in 3D Cell Culture and Their Phenotypic Analysis
July 12, 2023 03:00 PM (London)
Rüdiger Rudolf
Rüdiger Rudolf is Professor for Biosensorics at Mannheim University of Applied Sciences. He obtained his PhD in neural sciences from Heidelberg University, before his post-doc in the group of Prof. Tullio Pozzan (Padua/Italy), where he developed a combination of confocal and two-photon imaging in live mouse muscle. Later, as a group leader at the Karlsruhe Institute of Technology, he focused on sympathetic innervation and protein trafficking at neuromuscular junctions. In 2012, he joined Mannheim University, establishing a pipeline for 3D-cell cultures for applied pharmacological research. He's authored 80+ papers, cited over 10,000 times.
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Martin Fritsch
Dr. Martin Fritsch is a broadly trained biologist with a PhD in zoology. He undertook his postdoctoral research in evolutionary and developmental biology, focusing mainly on non-model organism invertebrates. Martin started his career at Leica in 2017, as a product sales specialist for CLSM. He changed to the advanced workflow specialist team in 2022, focusing on the advanced point scanning and fluorescence imaging instruments.
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Judith Reddington
Judith studied biomedicine at the University of Applied Sciences Bonn-Rhein-Sieg and the University of Aberdeen. For her doctorate she worked on gametogenesis at the University of Edinburgh. In 2012 Judith joined EMBL as a postdoc where she studied cell division at the beginning of life using the latest microscopy techniques. Her research was honored with the Paul Ehrlich and Ludwig Darmstaedter Prize for Young Researchers in 2020. In the same year Judith joined Leica Microsystems as product manager for volumetric imaging.
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Discover the exciting potential of 3D cell cultures in pharmacological research and explore innovative solutions to overcome challenges in generating, imaging, and analyzing these complex models.
In this webinar, you will discover:
- Potential solutions for phenotypic drug screening using 3D-cell models;
- Implications of Rayleigh scattering on 3D-microscopy;
- 3D-image segmentation and marker prediction.
3D cell cultures, such as spheroids and organoids, offer a model of intermediate complexity between classical adherent cell cultures and animal models and have been increasingly applied to pharmacological research.
However, their small size, three-dimensional organization, and biological characteristics constitute notable challenges during all major stages of phenotypic drug screens, including 3D-model generation, marker staining and drug exposure, live and fixed model 3D imaging, and data analysis.
At each of these levels, adequate compromises between biological (e.g., growth conditions, diffusion limits), physical (e.g., refractive index mismatches), and technological constraints (e.g., imaging depth and speed, data segmentation, and classification) need to be identified.
This webinar introduces an overview of the principal challenges in the planning and execution of a phenotypic drug screen and discusses potential solutions and respective drawbacks.
After the presentation, join Leica specialists for a live showcase demonstration of Leica’s Light Sheet (DLS) based on a STELLARIS imaging platform. Discover how DLS facilitates great penetration and image quality of spheroids due to dual-sided light sheet illumination and system-specific deconvolution algorithms; the ability to take advantage of the full excitation spectrum thanks to the STELLARIS white light laser. See for yourself how the unique combination of a confocal system and a light sheet microscope combined in the same system makes your research even more versatile.
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