Back to Buffer Basics: Everything You Need to Know About Buffers
Broadcast date: November 20, 2023On-demand
Thomas Warwick
Thomas has a Master's Degree in Chemistry from Loughborough University and a Ph.D. in Structural Biology from the University of Nottingham. After a brief period working as a Research Assistant in Knowledge Exchange, also at the University of Nottingham, Thomas became a Technician in Chemical Biophysics at Nottingham Trent University. In this role, he managed two research laboratories and provided technical and developmental guidance to students and researchers. In 2022, Thomas became a full-time content creator for Bitesize Bio, sharing his cross-disciplinary knowledge and research experience.
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Poorly prepared buffers cause failed Western blots, low protein yields, flat binding curves, and way more, so join us for a simple and clear explanation of how buffers work and how to prepare them.
In this webinar, you will learn:
- How buffers work;
- Considerations for choosing the best buffer system for your experiments to get reliable results;
- A method to correctly prepare buffer solutions in the lab.
Buffers are critical to nearly all our experiments. Poorly prepared buffers lead to:
❌ Failed Western blots.
❌ Poor protein yields.
❌ Flat binding curves.
❌ Wasted time.
They ensure that things run smoothly by maintaining the pH level in lab solutions, which is crucial to keep our target molecules stable and get reliable results.
Understanding why you need buffers, knowing how to choose the right one, and troubleshooting buffer issues are fundamental skills you can apply to make all your experiments work better—and you only need to learn how to prepare them correctly once.
In this webinar, we're diving deep into the world of buffers.
We'll explore how to select the best buffer for your experiments, share practical tips on preparing buffer solutions, and troubleshoot any hiccups that might come your way.
Plus, we'll demystify key concepts like pH and pI.
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