Aquilos 2 Cryo-FIB State-of-the-art Tomography Sample Preparation for In Situ Structural Biology

In this tutorial, you will find:

• How state-of-the-art cryo-FIB/SEM technology is used to produce suitable lamella samples from frozen-hydrated cells
• How cryo-tomography enables structural investigations of biomolecules in the context of cellular structures
• Advantages of cryo techniques for electron microscopy in preserving cellular samples in a close-to-native state

Cryo techniques for electron microscopy allow to preserve cellular samples in a close-to-native, fully hydrated state, frozen in non-crystalline vitreous ice. Utilizing a cryo-FIB microscope allows preparing site-specific thin sections, termed cryo-lamellae, from such vitrified objects. Cryo-lamellae are essentially snapshots of functional cellular environments whose supramolecular architecture can be studied in situ at high resolution and in 3D by electron tomography. The production of cryo-lamellae therefore enables structural investigations of biomolecules in the context of cellular structures. The webinar provides an introduction to cryo-tomography and explains how state-of-the-art cryo-FIB/SEM technology is used to produce suitable lamella samples from frozen-hydrated cells