Applying Cell Sorting in Clinical Immunology Research

Learn how cell sorting helps uncover disease-relevant signals hidden in complex immune populations, enabling more precise molecular and functional analysis in clinical immunology research.

In this webinar, you will learn:

• How cell sorting enables detection and validation of somatic mutations in autoimmune lymphoproliferative syndrome

• How isolated immune cell subsets can be used for functional and epigenetic validation of candidate variants such as TET2

• How cell sorting supports molecular characterization in large granular lymphocyte leukemia

• Practical insights into sorting strategies, purity, and downstream assay compatibility for immunology research and diagnostics

Cell sorting is a widely used and powerful technique for resolving cellular heterogeneity by isolating defined cell populations for downstream molecular and functional studies.

In immunology, this capability supports diverse applications, from probing immune cell function and differentiation to investigating immune-mediated disease.

In this webinar, Dr. Luis Allende presents real-world examples from a clinical immunology department to illustrate how cell sorting is applied in clinical diagnostics and translational research. The session begins with an overview of lymphoproliferative disorders in inborn errors of immunity, followed by three focused case studies.

You will see how cell sorting enables molecular characterization of somatic autoimmune lymphoproliferative syndrome (sALPS), supports functional validation of a TET2 candidate variant through epigenetic analysis, and aids the study of molecular variants in large granular lymphocyte leukemia (LGLL). Together, these examples demonstrate how isolating specific immune cell populations can uncover low-level somatic variants and clarify disease mechanisms.